Seven Direct Methods for Measuring HDL and LDL Cholesterol Compared with Ultracentrifugation Reference Measurement Procedures
نویسندگان
چکیده
منابع مشابه
Seven direct methods for measuring HDL and LDL cholesterol compared with ultracentrifugation reference measurement procedures.
BACKGROUND Methods from 7 manufacturers and 1 distributor for directly measuring HDL cholesterol (C) and LDL-C were evaluated for imprecision, trueness, total error, and specificity in nonfrozen serum samples. METHODS We performed each direct method according to the manufacturer's instructions, using a Roche/Hitachi 917 analyzer, and compared the results with those obtained with reference mea...
متن کاملLimitations of direct methods and the reference method for measuring HDL and LDL cholesterol.
Employment or Leadership: None declared. Consultant or Advisory Role: A.S. Jaffe, Beckman Coulter, Critical Diagnostics, Inverness Medical, Radiometer, Pfizer, and Amgen. Stock Ownership: None declared. Honoraria: A.S. Jaffe, Roche and Abbott Laboratories. Research Funding: A.K. Saenger, Roche Diagnostics; G.G. Klee, Beckman Coulter; A.S. Jaffe, Beckman Coulter. Expert Testimony: None declared....
متن کاملDirect Measurement of LDL Cholesterol
able amounts of radiolabeled dextran in the plasma protein fraction [15]. Consequently, we do not think this mechanism plays a substantial role in producing the lower than expected dextran concentrations seen in the present study. The dextran we used had a mean molecular mass of 70 000 Da. Thus, some quantity of lower-molecular-mass dextran could have leaked out of the vascular space and distri...
متن کاملEvolution of methods for measurement of HDL-cholesterol: from ultracentrifugation to homogeneous assays.
BACKGROUND Adoption of automated homogeneous assays for HDL-cholesterol (HDL-C) is increasing, driven by the need of clinical laboratories to cope with increasing workloads while containing costs. However, performance characteristics of homogeneous assays often differ in important aspects from those of the earlier precipitation methods. This review provides an overview of the new generation of ...
متن کاملComparison of a new method for the direct and simultaneous assessment of LDL- and HDL-cholesterol with ultracentrifugation and established methods.
BACKGROUND Automated electrophoresis combined with enzymatic cholesterol staining might improve routine assessment of LDL- and HDL-cholesterol (LDLC and HDLC), as an alternative to the Friedewald equation and precipitation. A new method (Hydrasys; SEBIA) that adapts the cholesterol esterase/cholesterol oxidase reaction within urea-free gels was evaluated. METHODS Fresh sera from 725 subjects ...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2010
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2009.142810